IB Biology SL Topic 3 — Adapting to the Environment Paper 1 & 2 Practical skill ~13 min read

Species Distribution (Skills)

You cannot count every plant on a beach, so you sample. And if you want to link a species to an abiotic factor, you cannot sample at random either — you have to sample along the thing that is changing. That is what a transect is for.

📚 What you need to know

Why not just sample at random?

Random sampling is the right tool when you want an unbiased estimate of how much of something is in an area. But that is not the question here. Here you want to know whether a species is more common where a factor is high than where it is low.

Scatter quadrats randomly across a sand dune and you get one average figure for the whole dune. Lay a line from the sea inland and sample along it, and you can see the species change as you go — because the salt, the water and the shelter all change as you go. The gradient is the whole point.

The rule of thumb Random sampling answers “how much?”
A transect answers “does it change along this gradient?”
Examiners often ask why a transect was used rather than random quadrats. The answer is always the same: the investigation is about an environmental gradient, and a transect deliberately samples across it.

The four types of transect

TypeWhat you recordBest for
Continuous line transectEvery species touching the tape, along its whole lengthShort transects where you want complete detail
Interrupted line transectSpecies touching the tape at set intervals, e.g. every metreLong transects; much quicker than continuous
Continuous belt transectQuadrats placed end to end along the whole lineDetailed abundance data over a short distance
Interrupted belt transectQuadrats placed at set intervals along the lineThe usual school choice: abundance data over a long gradient
Line transect and belt transect Both are shown here interrupted, with samples taken at regular intervalsLINE TRANSECT record every species touching the tape at each sample point sample pointBELT TRANSECT place a quadrat at each sample point and record what grows inside it quadratMake it continuous instead by sampling every point along the tape with no gaps.
The tape must run along the gradient, not across it. On a shore that means straight up the beach, not parallel to the water.

Measuring the abiotic factor

A transect that only records plants tells you a pattern with no explanation. You have to measure the abiotic factor at each sample point as well, or you cannot claim any link at all.

Only measure factors that are actually relevant. Water turbidity means nothing in a woodland, and there is no credit for recording it.

Abiotic factorHow you measure it
Air temperatureThermometer or temperature probe
Light intensityElectronic light meter
HumidityHygrometer, which reads the water vapour content of the air
RainfallRain gauge: a funnel collecting water into a measuring cylinder
Soil or water pHIndicator solution or a pH probe
Soil water contentWeigh a soil sample, dry it, weigh it again; the mass lost was water
Dissolved oxygenElectronic oxygen sensor
Water turbidityTurbidity meter, or lower a Secchi disc until it disappears and note the depth
Slope inclineClinometer, a protractor that lets the angle be worked out
Landscape relief (height of land)Contour lines on a map, or a GPS reading
Site aspect (which way it faces)Compass
Use sensors where you can. A data logger removes human judgement from the reading and lets you take many measurements quickly, which makes the data more reliable than eyeballing a scale in the wind.

Kite diagrams

A kite diagram shows distribution and abundance on the same picture. Each species gets its own horizontal strip. The shape sits symmetrically around a central line, spreading out where the species is common and pinching to nothing where it is absent.

🧩 How to read a kite diagram

  1. Each band is one species. Read the labels down the left first.
  2. Left to right is distance along the transect, given on the x axis.
  3. The width of the kite is abundance, usually percentage cover. Wider means more.
  4. It is symmetrical on purpose. The shape extends the same distance above and below the centre line, so half the total width is the actual value.
  5. Extra strips can show abiotic factors, such as elevation or pH, plotted against the same x axis.
Kite diagram for a sand dune transect Three species, plus the elevation of the dune, on one shared x axis elevation of the dune sea couch marram grass creeping willow 20% 0 20 40 60 80 100 distance from the high water line along the transect / mEach species peaks in a different place. That is the result.
Marram peaks on the dune crest where the sand is loosest and driest. Creeping willow appears only in the damp, sheltered hollow behind it.

🧠 The kite trap

The total height of a kite is twice the value. If a kite spans from 15% above to 15% below the line, the abundance is 30%, not 15%. Always check whether the scale is labelled on one side or across the whole width.

Worked examples

WORKED EXAMPLE

A 1 m by 1 m quadrat is divided into 100 equal squares. Marram grass fully covers 34 squares and covers about half of 12 more. Calculate the percentage cover. [2 marks]

Step 1: count the full squares 34 squares fully covered = 34 Step 2: count the part-covered squares as fractions 12 squares at about half each = 12 × 0.5 = 6 Step 3: add them, then convert to a percentage 34 + 6 = 40 squares out of 100 Percentage cover = 40% With 100 squares the count IS the percentage, which is exactly why quadrats are gridded that way.
WORKED EXAMPLE

Using the kite diagram above, state where marram grass is most abundant and suggest a reason for its distribution. [3 marks]

Step 1: read the widest point of the marram kite The marram kite is widest at about 30 m from the high water line, which matches the highest point of the dune. Step 2: quote the value Percentage cover there is about 26%, falling to zero beyond roughly 60 m. Step 3: give a biological reason The dune crest has loose, dry, salty, nutrient-poor sand. Marram is adapted to those conditions and faces very little competition there, so it dominates. Further inland the soil is damper and more stable, so other species outcompete it. Peaks at about 30 m at roughly 26% cover, on the exposed dune crest
WORKED EXAMPLE

Outline how you would investigate the effect of soil water content on the distribution of a plant species. [4 marks]

Step 1: set up along the gradient Lay a tape measure as a transect running from wet ground to dry ground, so the transect crosses the soil water gradient. Step 2: sample systematically Place a quadrat at regular intervals, for example every 2 m, and record the percentage cover of the species in each one. Step 3: measure the abiotic factor at the same points At each quadrat take a soil sample, weigh it, dry it in an oven, and weigh it again. The mass lost gives the soil water content. Step 4: repeat and present Run several parallel transects and take a mean, then plot the results as a kite diagram with a soil water strip on the same x axis. Transect along the gradient + regular quadrats + measure the factor + repeat The repeat step is the one people leave out, and it is nearly always worth a mark.

💡 Exam tip

⚠ Common mix-up

Up next: Abiotic Factors: Marine & Terrestrial — coral reefs, where a very narrow range of tolerance explains exactly why reefs sit where they do on a world map.

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