IB Biology HL Transport in Animals & Plants Paper 1 & 2 ~10 min read

Roots & Water Uptake

Most of the time water is pulled up a plant from the leaves. But on a still, humid night, or in early spring before the leaves have opened, there is no pull – and water still moves. The roots can generate a push of their own, and it starts with the plant spending energy on minerals.

📚 What you need to know

Getting water out of soil

Soil water is not free. It clings to soil particles, and it is a fairly dilute solution. To make water flow inwards, a root cell has to become a more concentrated solution than the soil – and it does that on purpose, by pumping minerals in.

From soil water to xylem xylem cortex cells root hair cell soil water and soil particles purple dots are mineral ions1 mineral ions are actively transported into the root hair cell 2 this lowers its water potential, so water enters by osmosis 3 ions and water cross the cortex and enter the xylem
Root hairs are long, thin extensions of an epidermal cell. Thousands of them give the root an enormous surface area for absorption.

How root pressure is generated

  1. Minerals are actively transported into the root cells from the soil. Active transport needs ATP, so the root has to respire to do it.
  2. The extra solutes lower the water potential of the root cells below that of the soil water.
  3. A water potential gradient now exists, so water moves in by osmosis.
  4. Minerals are actively transported from cell to cell across the root until they reach the xylem.
  5. As ions enter the xylem, its water potential falls, so water follows by osmosis.
  6. Water entering the xylem generates a positive pressure potential that pushes the water column upwards. This is root pressure.
The rule to hold on to more solutes → lower water potential → water moves in by osmosis
Watch the sign. Water potential of pure water is zero, and adding solute makes it more negative. So “lower water potential” means a more negative number, not a smaller amount of water. Students lose marks by writing “higher water potential” when they mean more concentrated.

Push versus pull

Two ways water moves up a stem DRY, SUNNY DAY STILL, HUMID NIGHT stomata open, water evaporates pulled from above, under tension little or no transpiration negative pressure in the xylem positive pressure in the xylem
Root pressure is much weaker than transpiration pull. It matters only when the pull has stopped – but at those times it is the only thing moving water.
Transpiration pullRoot pressure
Where the force comes fromEvaporation from the leavesActive transport of minerals into the root
Pressure in the xylemNegative – the column is under tensionPositive – the column is pushed
Needs ATP?No, it is driven by evaporationYes, active transport requires energy
StrengthStrong enough to lift water to the top of a treeWeak; moves water only a short distance
When it mattersWhenever stomata are open and air is drier than the leafWhen transpiration is low: high humidity, at night, or in early spring
Why waterlogged soil kills plants. Root cells need oxygen for aerobic respiration to make the ATP for active transport. In waterlogged soil there is little oxygen, so less ATP is made, fewer minerals are pumped in, the water potential gradient collapses and the plant takes up less water – even though it is standing in it.

Worked examples

WE 1

Generating root pressure

Explain how root pressure is generated in a plant. (4 marks)

Step 1: the pumping Mineral ions are actively transported from the soil into the root cells, using ATP. Step 2: the gradient This lowers the water potential of the root cells below that of the soil water, so water enters by osmosis. Step 3: reaching the xylem Ions are actively transported across the root into the xylem, lowering the water potential there, so water follows by osmosis. Step 4: the push Water entering the xylem creates a positive pressure potential that pushes the column of water upwards. Pump the ions, the water follows, and the pressure pushes every step involves a water potential gradient – say so each time
WE 2

Roots without oxygen

Explain why a plant in waterlogged soil may absorb less water than a plant in well-drained soil. (3 marks)

Point 1: the shortage Waterlogged soil contains very little oxygen, so root cells cannot respire aerobically at a normal rate. Point 2: the energy Less ATP is available, so fewer mineral ions can be actively transported into the root cells. Point 3: the consequence The water potential of the root cells does not fall far below that of the soil, so the gradient is small and less water enters by osmosis. No oxygen means no ATP, no ion pumping and no gradient an excellent example of how an animal-style respiration point turns up in a plant question
WE 3

When root pressure matters

Suggest why root pressure is more important in a deciduous tree in early spring than in midsummer. (3 marks)

Point 1: early spring The leaves have not yet developed, so there is little or no transpiration and therefore no transpiration pull. Point 2: what takes over Root pressure can still push water up the xylem, because it depends on active transport in the root rather than on evaporation from leaves. Point 3: midsummer Once the leaves are open, transpiration pull is far stronger and moves most of the water, so the contribution of root pressure is comparatively small. No leaves means no pull, so the push is all there is the same reasoning applies at night and in very humid conditions

💡 Exam tips

⚠ Common mistakes

Up next: Translocation in Plants – the other transport tissue, and the only one that can move its cargo in either direction.

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