IB Biology HL Natural Selection Paper 1 & 2 ~10 min read

Gene Pools

Individuals are temporary. Alleles are not. Zoom out from the single organism and a population looks like a shared pot of alleles that gets shuffled and dealt again every generation — and evolution is simply that pot slowly changing its contents.

📚 What you need to know

What a gene pool is

Definition A gene pool consists of all the genes, and all their different alleles, present in an interbreeding population

The word interbreeding is doing a lot of work in that sentence. Two organisms only share a gene pool if their alleles can end up in the same offspring. Squirrels on opposite sides of an ocean belong to the same species but not to the same gene pool.

Thinking about all the alleles in a population at once matters because those alleles are exactly what the next generation will be built from. Nothing else is available. If an allele is missing from the pool, no offspring can inherit it, no matter how useful it would have been.

One species can have several gene pools

Some populations of the same species are geographically isolated from each other — separated by a mountain range, a stretch of sea, a motorway. Because they cannot interbreed, multiple gene pools can exist for a single species, and those pools can drift apart in composition over time.

One species, two gene pools Each circle is one copy of an allele carried by the population B B b B B B b B B B b B b B b b b B B b b b B bPOPULATION A B frequency 0.75 POPULATION B B frequency 0.33 barrierSame species, same alleles, very different frequencies Once the two pools stop mixing, they are free to drift apart
Twelve allele copies in each pool here. In a real population it would be thousands, but the idea is identical.

When is a gene pool stable?

A population retains a stable gene pool — meaning allele frequencies stay the same from generation to generation — under four conditions.

Here is the sentence that ties this whole topic together:

The link to remember A stable gene pool means the population is not evolving. If allele frequencies are changing, the population is evolving.
Read those four conditions again and notice how unrealistic they are. Real populations are not infinitely large, mating is rarely random, and something is always acting as a selection pressure. That is the point — stability is the theoretical baseline you compare real populations against, which is exactly how the Hardy-Weinberg principle is used later in this topic.

What changes allele frequencies

Darwin came up with the theory of evolution by natural selection without any knowledge of genetics or DNA. His theory has since been developed by modern scientists who added genetics to it, and the combined version is called neo-Darwinism. That is what lets us describe evolution at the molecular level, as a change in allele frequency in a gene pool.

Three processes do the changing.

ProcessWhat drives itIs it random?
Natural selectionSome phenotypes survive and reproduce more because they are better adaptedNo — the environment decides which alleles do well
Sexual selectionSome phenotypes are chosen as mates more oftenNo — the opposite sex decides
Genetic driftChance events change which individuals happen to breed or surviveYes — entirely down to luck

Genetic drift in plain words

Imagine a population of twenty beetles, half green and half brown, in a habitat where colour makes no difference at all. A falling rock crushes six green beetles. Nothing about being green caused that — they were simply standing in the wrong place. The next generation is still built from whoever is left, so the frequency of the green allele drops anyway.

That is genetic drift: a change in allele frequency caused purely by chance. It matters most in small populations, where one unlucky event affects a large fraction of the pool. In a population of a million beetles, six deaths change nothing measurable.

Evolution, measured properly A gene pool under selection: the frequency of one allele climbing over four generations 0.5 0.30 0.45 0.62 0.80Gen 1 Gen 2 Gen 3 Gen 4 frequency of allele BThis population is evolving Four flat bars of equal height would mean a stable gene pool and no evolution
You cannot see evolution by looking at one organism. You see it by counting alleles in a population across generations.
Careful with the word “random”. Mutation is random. Genetic drift is random. Natural selection is not. It is the one step in the whole process that is directed, because the environment consistently favours the same phenotypes.

Worked examples

WE 1

Gene pools of an isolated population

A river changes course and divides a population of insects into two groups that can no longer meet. Explain why the two groups may develop different allele frequencies. (3 marks)

Point 1: two gene pools The groups no longer interbreed, so alleles cannot be exchanged and there are now two separate gene pools. Point 2: different pressures The two sides of the river may have different selection pressures, so different phenotypes survive and reproduce on each side. Point 3: chance Genetic drift also acts independently in each group, especially if either population is small. No gene flow, so the pools change independently if the isolation lasts long enough this can lead to speciation, which is worth naming
WE 2

Deciding whether a population is evolving

The frequency of an allele in a beetle population is 0.42 in one generation and 0.41 in the next. State, with a reason, whether the population is evolving. (2 marks)

Statement The allele frequency has changed, so by definition the population is evolving. Reason with a caution The change is very small, so it could be caused by genetic drift or by sampling error rather than by selection. More generations of data would be needed to identify a trend. Any change in allele frequency is evolution — but not all change is selection the second mark comes from being cautious, not from being certain
WE 3

Why small populations are vulnerable

A conservation team is worried that a rare bird population has fallen to 30 individuals. Explain why a small gene pool is a problem. (3 marks)

Point 1: drift is strong In a small population, chance events change allele frequencies quickly, so alleles can be lost from the gene pool entirely. Point 2: less variation A smaller gene pool contains fewer different alleles, so there is less variation for selection to act on. Point 3: the consequence If the environment changes, no individual may carry a useful allele, so the population cannot adapt and may become extinct. Small pool, fast drift, low variation, poor chance of adapting this is why conservation programmes move individuals between reserves — to keep the gene pools mixed

💡 Exam tips

⚠ Common mistakes

Up next: Allele Frequencies (Skills). Now that you know what a gene pool is, the next step is counting one — working out actual frequencies from a list of genotypes, and seeing what happens when a handful of individuals start a brand new population.

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