IB Biology HL Skill Set 3 — Maths for Biology Paper 1, 2 & IA Core skill ~12 min read

Maths Skills for Biology

Nobody fails Biology because the maths is hard. The sums here are the ones you did at 14. People lose marks because they pick the wrong average, use the wrong percentage formula, or forget what the number is actually telling them about the organism.

📚 What you need to know

The maths you will actually use

Every one of these turns up somewhere in the course. It helps to know where, because then the maths stops feeling random.

Type of numberWhere it shows up in Biology
DecimalsAlmost every measurement — the length of a bacterial cell, the mass of a seedling
FractionsYour calculator often gives an answer as a fraction. Find the button that flips it to a decimal before the exam, not during it
PercentagesPercentage change in mass in an osmosis practical, percentage cover in a quadrat
RatiosSurface area to volume ratio — the one ratio you must be fluent with
ProportionsScaling a drawing up or down from a micrograph so the shape stays true
FrequenciesAllele frequency in a population, and how it shifts over generations
DensitiesPopulation density in ecology, stomatal density on a leaf
ApproximationsQuick estimates, such as checking a magnification answer looks sensible
ReciprocalsTurning a time into a rate: rate = 1 ÷ time taken
The reciprocal trick catches people out. If a colour change takes 50 seconds, the rate is 1/50 = 0.02 s−1. A faster reaction takes less time, so it gets a bigger rate. That is exactly why we flip it — a graph of time looks upside down compared to a graph of rate.

Averages: mean, median and mode

These three all try to answer the same question — “what is a typical value here?” — but they answer it in different ways.

AverageHow you find itWhat it is good for
MeanAdd up all the values, divide by how many there areSumming up a whole data set in one number. It uses every value, so every value affects it
MedianPut the values in order and take the middle oneData with an odd result that would drag the mean off. Half the values sit below it, half above
ModeThe value that appears most oftenCategories, where a mean makes no sense — the most common blood group, the most common eye colour
One odd result changes the mean, not the median dandelions counted in nine quadrats on the same field 0 4 8 12 16 20 24 median and mode = 5 mean = 6.7 one huge count drags the mean upnumber of dandelions per quadrat
Eight quadrats gave counts between 3 and 7. The mean says 6.7, which describes none of them. The median, 5, describes the field far better.
If a question gives you a data set with one value miles away from the rest, the examiner usually wants you to notice it. Say the mean is affected by the outlier and that the median is more representative here. That one sentence is often the mark.

Spread: how tightly packed is the data?

A mean on its own is only half a story. Two sets of results can have exactly the same mean and still be nothing alike. That is what the measures of dispersion are for.

Same mean, very different spread two groups of five leaves, both with a mean length of 20 mm mean = 20 mm for bothGroup A SD = 1.6 mmGroup B SD = 7.9 mm 10 15 20 25 30 leaf length / mm
Report a mean without a spread and you have hidden the difference between these two groups completely.
MeasureWhat it tells youWatch out for
Standard deviation (SD)How far the values sit from the mean on average. Small SD means the results are consistentYou must work out the mean first. Useful for comparing how consistent two data sets are
Standard error (SE)How far your sample mean is likely to be from the true mean of the whole populationIt is about how good your sample is, not how varied the organisms are. SE is always smaller than SD
Interquartile range (IQR)The difference between the 75th and 25th percentiles — the range of the middle half of the dataQuartiles cut the ordered data into four equal parts. The IQR ignores extreme values completely
🧠

SD or SE?

SD = how Different the individuals are from each other. SE = how Exact your estimate of the mean is. If you sample more organisms, SE shrinks, but SD does not have to.

Scientific notation

Biology jumps between the size of a ribosome and the size of a rainforest, so you need a tidy way to write very big and very small numbers. Scientific notation, also called standard form, does that.

Standard form a × 10n
Quick check. 0.0000000001 m is 1 × 10−10 m, roughly the width of an atom. 602 000 000 000 000 000 000 000 is 6.02 × 1023, the number of particles in a mole. Same numbers, far less ink.

Approximation and estimation

These two words sound identical and get used loosely, but the exam treats them as different jobs.

The classic biological estimate is the age of the first cells and of LUCA. No fossil has a date stamped on it, so biologists count mutations and run a molecular clock instead. That is estimation, and it is why those dates are always given as “about”.

Rates of change

A rate tells you how fast something changes. In Biology it is nearly always “how much, per unit of time”.

Average rate of change rate = change in the dependent variable ÷ change in the independent variable

If you have a table, subtract one row from another. If you have a graph, the rate is the gradient. Both give the same thing, so use whichever the question hands you.

WE 1

Calculate a rate from a table

A respirometer measured the oxygen used by germinating seeds. At 2 minutes, 0.8 cm3 had been used. At 10 minutes, 3.2 cm3 had been used. Calculate the average rate of oxygen uptake. (2 marks)

Step 1: find both changes change in volume = 3.2 − 0.8 = 2.4 cm3 change in time = 10 − 2 = 8 min Step 2: divide, and keep the units rate = 2.4 ÷ 8 = 0.30 0.30 cm3 min−1 the unit is a giveaway mark — “per minute” comes straight from the bottom of the fraction

Describing trends: proportionality and correlation

Examiners are fussy about these words, so use them precisely.

TermWhat it means
Directly proportionalA straight-line relationship: if x doubles, y doubles. As one goes up, so does the other, by the same factor
Inversely proportionalIf x doubles, y halves. As one goes up, the other comes down
Positive correlationThe graph slopes upwards: as x increases, y increases. It does not have to be a straight line
Negative correlationThe graph slopes downwards: as x increases, y decreases
Not the same thing. Every directly proportional relationship is a positive correlation, but plenty of positive correlations are not proportional. “Proportional” is a strong claim — only use it for a straight line that heads for the origin.

Percentage change and percentage difference

This is where most of the lost marks live. Both compare two numbers, but they answer different questions, and they use different formulas.

Percentage change

Use this when one value came before the other — a starting mass and a final mass, a count before treatment and after.

Percentage change (final value − initial value) ÷ initial value × 100

Divide by the initial value, because that is the thing you are comparing against. A negative answer simply means it went down, and you should say so.

Percentage difference

Use this when you are comparing two values that are just… two values. There are two versions, and the question decides which one you need.

Method 1 — relative change (number A − number B) ÷ number A × 100
Method 2 — symmetrical difference (number A − number B) ÷ [(number A + number B) ÷ 2] × 100

Method 1 treats A as the baseline, so it answers “how much bigger is B compared with A?”. Method 2 divides by the mean of the two numbers instead, so it does not matter which one you call A. Use it when neither value is a starting point.

Which percentage formula? Is there a clear starting value that the other number is compared to? YES NO PERCENTAGE CHANGE divide by the initial value e.g. mass of a potato chip before and after PERCENTAGE DIFFERENCE divide by the mean of the two e.g. two unrelated means from a tableRead the wording twice before you choose.
The words “increased to”, “after” and “originally” all point at percentage change. Words like “compare” with no before-and-after point at percentage difference.
WE 2

Percentage change in an osmosis practical

A potato cylinder had a mass of 5.20 g. After 30 minutes in a sucrose solution its mass was 4.42 g. Calculate the percentage change in mass. (2 marks)

Step 1: there is a clear starting mass, so use percentage change change = 4.42 − 5.20 = −0.78 g Step 2: divide by the initial mass and multiply by 100 (−0.78 ÷ 5.20) × 100 = −15.0 −15.0 % (a 15 % loss in mass) keep the minus sign and say what it means — water left the cells, so the solution was more concentrated than the cell sap
WE 3

Symmetrical percentage difference

A student counted stomata on two leaf surfaces. The mean was 12 stomata per mm2 on the upper surface and 68 per mm2 on the lower surface. Calculate the percentage difference between them, to three significant figures. (3 marks)

Step 1: decide on the method Neither surface is a starting value, so there is no baseline — use the symmetrical formula. Step 2: find the difference 68 − 12 = 56 Step 3: find the mean of the two values (68 + 12) ÷ 2 = 40 Step 4: divide and multiply by 100 (56 ÷ 40) × 100 = 140 140 % difference a percentage over 100 is fine here — the gap between the two values is bigger than their average

Discrete and continuous data

Which type you have decides how you graph it and which test you can use, so sort this out early.

TypeWhat it isBiological examples
DiscreteQuantitative data made of separate, countable values. You cannot have half of oneNumber of woodlice in a sample, number of offspring in a litter
ContinuousQuantitative data from measuring. It can take any value in a range, including decimalsTemperature of an enzyme reaction over time, volume of oxygen released by pondweed

Choosing a statistical test

You will be given the formulas in the exam. What you have to supply is the judgement about which one fits.

Test or indexUse it when you want to…
t-testCompare the means of two sets of data and decide whether the difference is significant. The data should be continuous, roughly normally distributed, with similar standard deviations
Chi-squared testCompare observed results with expected results — the outcome of a genetic cross, or whether two species are associated
Correlation testFind out whether two variables are related, and how strongly
Simpson’s reciprocal indexMeasure the biodiversity of a community. It takes in both the number of species (richness) and how many individuals of each (evenness). A higher value means more biodiversity
Lincoln indexEstimate the size of an animal population by capture, mark, release and recapture

Three things push you towards one test rather than another: the size of the sample, whether the data is discrete or continuous, and what the question is actually asking.

💡 Exam tips

⚠ Common mistakes

Up next: Units, Symbols & Values — the SI base units, the prefixes from nano to kilo, and the significant figure rules that decide how your answer is written down.

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