IB Biology HL Stage 2 — Collect & Process Data IA & Paper 2 Practical skill ~12 min read

Collecting Data

This is the doing phase — the part where you finally run the experiment you spent so long designing. The job now is to come away with raw data that is honest, precise and complete, including the things you noticed as well as the things you measured.

📚 What you need to know

The raw data table

The raw data table is the first table in your report and the one everything else is built from. If it is wrong, every calculation after it inherits the problem.

What a raw data table has to have design it before you start, not after the practicalTable 1: effect of temperature on pigment released from beetroot Temperature / °C (±0.5) Absorbance trial 1 (±0.01) Absorbance trial 2 (±0.01) Absorbance trial 3 (±0.01)20 0.07 0.09 0.08 30 0.10 0.12 0.11 40 0.17 0.20 0.20 a specific title not just “Results” units and uncertainty live in the header raw readings only no calculations hereevery value written to the precision of the instrument
The independent variable goes in the left-hand column, in order. Each replicate gets its own column, so nothing is averaged before it has been written down.
RequirementWhat it looks like in practice
A specific titleIt names both variables and the organism, not just “Results table”
Labelled columnsThe independent variable on the left, then one column per replicate of the dependent variable
Units and uncertainties in the header“Time / s (±0.2)” at the top, so the body of the table holds nothing but numbers
Direct measurements onlyInitial and final mass, not the change in mass. The change is a calculation and belongs in the next table
Enough dataAt least five values of the IV, at least three replicates each, and five replicates if you can manage it
Doing the sums in your head as you go, and only writing down the answer, is the fastest way to lose marks in this section. The assessor cannot check a calculation they cannot see, and if you have thrown away the original readings there is no way back.

Recording to the right precision

Your instrument decides how many decimal places you write. Writing fewer throws away information you actually collected; writing more claims precision you never had.

Record to the precision of the instrument one of the easiest places on the whole report to drop a mark 1.50 g 24.5 cm³ 24.63 sbalance reads to 0.01 g 1 cm³ divisions stopwatch reads to 0.01 swrite 1.50 g ✓ read to nearest 0.5 ✓ write 24.63 s ✓ not 1.5 g ✗ not 24 cm³ ✗ not 25 s ✗The instrument decides how many decimal places you write.
The trailing zero in 1.50 g is not decoration. It says the balance could tell the difference between 1.50 and 1.51, and that is real information.
Every value in one column, the same way. If one mass is 1.50 g, none of the others may be written as 1.5 g. A column with mixed precision looks careless even when the readings are right.

Qualitative observations

Qualitative data is everything you noticed but could not put a number on. It is not filler, and it is not an afterthought. It is often the only thing that explains a result which otherwise looks like a mistake.

Type of observationExampleWhat it can explain later
Turgidity of plant tissuePotato cylinders feeling firm and stiff, or soft and limpWhether water moved in or out, backing up your mass data
ColourLeaves yellowing from a mineral deficiencyWhy a plant’s growth rate was lower than expected
Behavioural responseWoodlice moving steadily away from a light sourceThe direction of a response, not just the count in each half
Texture or appearanceA cloudy milk suspension turning clearHow far a reaction actually went, and whether it finished at all
Here is the pattern that earns marks later. Your numbers say the mass gain in pure water was smaller than expected. Your observation says the cylinders already felt soft and floppy at the start. Put the two together and you have a real explanation: the tissue was partly dehydrated before you began.

When the experiment misbehaves

Biological experiments rarely run perfectly. Noticing a problem and responding to it sensibly is a scientific skill in itself — and it only counts if you write it down.

What to do when a reading looks wrong the response gets recorded, never hidden STEP 1 STEP 2 STEP 3 STEP 4you spot a reading that does not fit write it into the table anyway run one extra trial at that value keep both, and justify it later Never rub out an outlier. You justify excluding it later, when you process.
The extra trial is what turns a suspicion into evidence. Two readings agreeing and one disagreeing is a much stronger case than one odd number on its own.
ProblemHow to respond
The reaction is far too fast or too slowAdjust the enzyme or substrate concentration until the rate is measurable, then record the change you made and the reason for it
One repeat gives a wildly different resultRecord it, then run an additional trial so you end up with a set of concordant results. Do not delete anything
Organisms are not behaving as expectedIn fieldwork, empty quadrats may mean you need to reconsider the sampling location. Record that decision and your reasoning
A sample is prepared wronglyDiscard it and prepare a replacement before starting, so every sample really is comparable. Note what happened
WE 1

Designing the header row

A student will time how long a filter paper disc soaked in catalase takes to rise in hydrogen peroxide, at five concentrations, with three repeats. Write a suitable set of column headers for the raw data table. (3 marks)

Step 1: independent variable on the left, with unit and uncertainty Concentration of hydrogen peroxide / % (±0.05) Step 2: one column per replicate of the dependent variable Time for disc to rise, trial 1 / s (±0.2) Time for disc to rise, trial 2 / s (±0.2) Time for disc to rise, trial 3 / s (±0.2) Step 3: check what is missing No mean column, and no rate column — those are calculations, so they belong in the processed table. Four columns, units and uncertainties in the headers, no calculated values the ±0.2 s allows for reaction time, not just the stopwatch display — say so if asked
WE 2

Correcting how readings were recorded

A student records these masses from a balance that reads to two decimal places: 2.4 g, 2.37 g, 2.400 g. Explain what is wrong and rewrite them. (3 marks)

Problem 1: too few decimal places 2.4 g throws away a digit the balance actually gave, and it does not match the rest of the column. Problem 2: too many decimal places 2.400 g claims the balance can read to 0.001 g, which it cannot. That is dishonest precision. Step 3: match every value to the instrument All three must be written to two decimal places. 2.40 g, 2.37 g, 2.40 g consistency down a column is the quick check — every entry should have the same number of decimal places
WE 3

Responding to an odd reading

In the beetroot investigation, the three absorbance readings at 50 °C are 0.40, 0.71 and 0.43. Describe how the student should respond, during the practical. (3 marks)

Step 1: record it as it stands Write 0.71 into the table and mark it as possibly anomalous. Do not erase it. Step 2: get more evidence Run a fourth trial at 50 °C. It gives 0.43, which agrees with the other two. Step 3: note a possible cause Record a likely reason — for example the disc was cut from tissue that had already been damaged, releasing extra pigment before the water bath. Record it, run a fourth trial, note the probable cause you do not exclude it yet — that decision happens on the next page, and it has to be justified

💡 Exam tips

⚠ Common mistakes

Up next: Processing Data — turning those raw readings into means, rates and standard deviations, with the working shown and the significant figures under control.

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