IB Biology SL Inquiry Stage 3 — Conclude & Evaluate Internal assessment Core skill ~10 min read

Evaluating the Method

The evaluation is where you turn on your own work. Not to apologise for it — to show you understand what your method could and could not deliver. The mark is not for finding faults; it is for tracing what each fault did to your result and suggesting a fix that would actually work in a school lab.

📘 What you need to know

Start by evaluating your hypothesis

Your conclusion said whether the data supported the hypothesis. The evaluation asks a harder question: how strongly, given everything you now know about your data.

Even a supported hypothesis can rest on shaky ground. If the standard deviation at 50 °C was double that anywhere else, the exact point where denaturation sets in is less certain than the neat curve suggests — and saying so is a stronger piece of writing than claiming the graph settled it.

The two kinds of error

Telling these apart matters, because they do different damage and they need different fixes.

What each kind of error does to your graph SYSTEMATIC RANDOM every point shifted the same way points scattered either side repeats will not reveal it repeats average it out The faint grey line is the true relationship in both cases.
Look at the shape of the left-hand curve: it still peaks in the right place. A systematic error can leave your trend intact while making every value wrong.
TypeWhat it looks like in your dataBiology examplesHow to reduce it
SystematicEvery reading off in the same direction; repeats agree with each other but not with realityA water bath running 2 °C below its setting; a balance never tared; surface water left on tissue before weighingCalibrate instruments; improve the technique; add a control
RandomReadings scatter above and below; large standard deviationsNatural variation between organisms; reaction time on a stopwatch; judging a colour change by eyeMore repeats and a mean; a more objective measurement
The biology-specific one: natural variability between living samples is the single most common source of random error in a biology investigation, and it is worth discussing properly. Two leaves from the same plant are not identical, and no amount of careful technique changes that.

Weakness, limitation, assumption

These three get lumped together, and separating them is an easy way to make an evaluation look organised.

Three different things to write about WEAKNESS LIMITATION ASSUMPTION a flaw in the method that caused error restricts how widely your conclusion applies a simplification you made and never tested judging the end point of a colour change by eye only one enzyme source was tested, at one pH that the buffer held pH constant right through the run A weakness needs a fix. A limitation needs a wider study. An assumption needs a check. Naming which of the three you are discussing makes the evaluation much easier to follow.
Limitations are not failings. Every investigation is limited — the skill is knowing exactly where your conclusion stops being safe.

Close the loop every time

This is the structure that separates a strong evaluation from a list of complaints. Each point needs all three parts, and the middle one is where most marks are won and lost.

Weakness, impact, improvement — all three, every time WEAKNESS IMPACT IMPROVEMENT what exactly was wrong with the step which way it pushed your actual result specific, and doable in a school lab The middle box is the one students skip, and it carries the most credit. Say which way the error pushed your numbers: too high, too low, more scattered. Buy better equipment is not an improvement. Neither is be more careful next time.
“There may have been errors in the timing” is a weakness with no impact and no fix. It reads as filler, because it is.
WORKED EXAMPLE

In the amylase investigation the end point was judged by eye — when the iodine stopped turning blue-black. Evaluate this as a weakness.

Weakness The end point was judged by eye, and the colour faded gradually rather than disappearing at one clear moment. This is a systematic error, because the same person tends to call the end point at the same slightly late stage every time. Impact on the result Every recorded time is slightly too long, so every calculated rate is slightly too low. The whole curve is shifted down, though the position of the optimum at 40 °C is unaffected because the shift applies to all temperatures. It also made the readings at 10 and 50 °C, where clearing was slowest, harder to judge and more scattered. Improvement Use a colorimeter with a fixed filter and stop timing at a set absorbance value, or record with a data logger so the end point is defined by a number rather than a judgement. Named error type, direction of the shift, specific fix Notice the impact says which way the numbers moved. “It made the results less accurate” would say almost nothing.
WORKED EXAMPLE

Evaluate the variability of the enzyme and starch solutions as a source of random error.

Weakness Small differences in mixing, in the time taken to transfer the tube into the water bath, and in how long solutions had stood before use, varied unpredictably between trials. This is a random error. Impact on the result It scattered the repeat times either side of the true value, producing the standard deviations of 1.4–4.0 s. The largest spread was at 50 °C, which widened the error bars exactly where the falling section of the curve needed to be read most carefully. Improvement Pre-warm the enzyme and substrate separately in the water bath for a fixed 10 minutes before mixing, start the timer at the moment of mixing, and raise the number of repeats from three to five so the mean is less affected by any one trial. Random error, quantified from your own data, with a workable fix Quoting your own standard deviations is what makes this an evaluation of your experiment rather than a generic paragraph.

Limitations and assumptions

These are not errors. They are the boundaries around your conclusion, and stating them shows you know exactly how far your result reaches.

WORKED EXAMPLE

Give one limitation and one assumption for the amylase investigation, with an improvement or check for each.

Limitation Only one source of amylase was tested, at a single pH, over a 40 °C range in 10 °C steps. So: the conclusion that the optimum is 40 °C applies to this enzyme under these conditions, and cannot be generalised to amylase from other organisms. The 10 °C steps also mean the true optimum could lie anywhere between about 35 and 45 °C. Extension: repeat with 2 °C steps between 30 and 50 °C to locate the optimum more precisely. Assumption It was assumed the buffer held the pH constant throughout, and that the tubes reached the set temperature before the enzyme was added. Check: measure the pH of each tube at the start and end with a calibrated meter, and use a thermometer in a dummy tube to confirm the contents had reached temperature. Scope stated, and a way to test what you assumed A limitation naturally suggests the next investigation, which is a good place for an evaluation to end.

Writing it well

A quick self-test: read your evaluation and see whether swapping in a different experiment’s title would still make it true. If it would, it is too generic to score.

💡 Exam tip

⚠ Common mix-up

That completes the inquiry cycle. Up next: Exploring a Problem — because a good evaluation always ends by pointing at the next investigation, and the cycle starts again.

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