IB Biology SL Gas Exchange Systems Paper 1 & 2 Practical skill ~11 min read

Measuring Lung Volumes

A spirometer turns breathing into a wiggly line, and almost every mark in this topic comes from reading that line correctly. The four measurements you need are all just vertical distances on the same graph — once you know where to put the ruler, they become straightforward.

📚 What you need to know

The apparatus

🤔 Why soda lime changes what you are measuring

Without soda lime, every breath returns roughly the same total volume of gas to the chamber — oxygen is removed by the body but carbon dioxide is added back, so the overall volume barely drops. Put soda lime in the circuit and the carbon dioxide is absorbed instead of returning. Now the total gas in the chamber falls steadily, and the rate of that fall is the rate of oxygen consumption. Same machine, one chemical, completely different measurement.

Reading a spirometer trace

Every measurement is a vertical distance Quiet breathing, then one deep breath in and right out, then quiet breathing again. 0 1 2 3 4 5 6 0 10 20 30 40 maximum inspiratory level maximum expiratory level TV = 0.5 IRV = 2.5 ERV = 1.3 VC = 4.3 volume of air in lungs / dm³ time / s Check it: 0.5 + 2.5 + 1.3 = 4.3, which is the vital capacity. If your three values do not add up to VC, one of them has been read off wrongly.
The y-axis is the volume of air in the lungs, not the volume breathed. That is why the line never reaches zero — you cannot empty your lungs completely.

The four measurements

MeasurementHow to read it off the traceOn this trace
Tidal volume (TV)The height of one normal quiet breath, from trough to peak3.0 − 2.5 = 0.5 dm³
Inspiratory reserve volume (IRV)From the top of a normal breath up to the maximum inspiratory level5.5 − 3.0 = 2.5 dm³
Expiratory reserve volume (ERV)From the bottom of a normal breath down to the maximum expiratory level2.5 − 1.2 = 1.3 dm³
Vital capacity (VC)Maximum inspiratory level down to maximum expiratory level, in one breath5.5 − 1.2 = 4.3 dm³
Ventilation rateCount the peaks in a known time and scale to one minutePeaks every 4 s, so 15 breaths per minute
The equation to memorise VC = TV + IRV + ERV
Use that equation as a check, not just as a formula. If a question gives you three of the four values, the fourth is free marks. If it gives you all four and they do not add up, you have misread the graph.

What exercise does to the trace

A useful extra number: total ventilation per minute is tidal volume × ventilation rate. For the trace above that is 0.5 dm³ × 15 breaths = 7.5 dm³ of air per minute. Under hard exercise both factors rise, so the total can climb many times higher.

Worked examples

WORKED EXAMPLE

A person’s trace shows a tidal volume of 0.45 dm³, an inspiratory reserve volume of 2.80 dm³ and an expiratory reserve volume of 1.15 dm³. Calculate the vital capacity. [2]

Step 1: recall the equation VC = TV + IRV + ERV Step 2: substitute VC = 0.45 + 2.80 + 1.15 VC = 4.40 dm³ Keep the units. A number with no units usually drops a mark.
WORKED EXAMPLE

On a spirometer trace, 9 complete breaths occur in 36 seconds and the tidal volume is 0.55 dm³. Calculate the ventilation rate and the volume of air breathed per minute. [3]

Step 1: breaths per second 9 ÷ 36 = 0.25 breaths per second Step 2: scale to one minute 0.25 × 60 = 15 breaths per minute Ventilation rate = 15 breaths per minute Step 3: total volume per minute 0.55 × 15 = 8.25 dm³ 8.25 dm³ of air per minute
WORKED EXAMPLE

Describe two changes you would expect to see in a spirometer trace taken during exercise compared with one taken at rest. [2]

Change 1 The peaks and troughs are further apart vertically, showing an increased tidal volume. Change 2 The peaks are closer together horizontally, showing an increased ventilation rate Do not write “vital capacity increases” — it does not.

💡 Exam tip

⚠ Common mix-up

Up next: Gas Exchange in Plants — the same physics, but with a problem animals do not have: every gas that goes in lets water out.

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